Rhodanese


Rhodanese, also known as rhodanase, thiosulfate sulfurtransferase, thiosulfate cyanide transsulfurase, and thiosulfate thiotransferase, is a mitochondrial enzyme that detoxifies cyanide by converting it to thiocyanate.
This reaction takes place in two steps. The diagram on the right shows the crystallographically-determined structure of rhodanese. In the first step, thiosulfate is reduced by the thiol group on cysteine-247 1, to form a persulfide and a sulfite 2. In the second step, the persulfide reacts with cyanide to produce thiocyanate, re-generating the cysteine thiol 1.
This reaction is important for the treatment of exposure to cyanide, since the thiocyanate formed is around 1 / 200 as toxic.:p. 15938 The use of thiosulfate solution as an antidote for cyanide poisoning is based on the activation of this enzymatic cycle.
Rhodanese shares evolutionary relationship with a large family of proteins, including
Rhodanese has an internal duplication. This domain is found as a single copy in other proteins, including phosphatases and ubiquitin C-terminal hydrolases.

Human proteins containing this domain

; CDC25B; CDC25C; DUSP; DUSP1; DUSP10; DUSP16;
DUSP2; DUSP4; DUSP5; DUSP6; DUSP7; KAT; MKP7; MOCS3;
MPST; TBCK; TSGA14; TST; USP8;

Nomenclature

Although the standard nomenclature rules for enzymes indicate that their names are to end with the letters "-ase", rhodanese was first described in 1933, prior to the 1955 establishment of the Enzyme Commission; as such, the older name had already attained widespread usage.

Thiosulfate sulfurtransferase

In enzymology, a thiosulfate sulfurtransferase is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are thiosulfate and cyanide, whereas its two products are sulfite and thiocyanate.

Nomenclature

This enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is thiosulfate:cyanide sulfurtransferase. Other names in common use include thiosulfate cyanide transsulfurase, thiosulfate thiotransferase, rhodanese, and rhodanase.