He was born in Naples, but left Italy around 1640 for unknown reasons, perhaps simply while following a troupe of actors, or to flee a political intrigue. He arrived in France under the reign of Louis XIII. His acting pleased the queen, which enabled him to attend the court. It is said that one day, when the two-year-old Dauphin cried, Fiorilli, as Scaramouche, made any possible sound to comfort him. He achieved this task with grimaces and tomfoolery; consequently, the Dauphin had "a need, that he had at the time, the hands and the dress of Scaramouche". Fiorilli was then ordered to visit the court every night to amuse the Dauphin, which helped the Scaramouche character become a stock figure in the theatre of the time. The character of Scaramouche became a standard role thereafter of the commedia dell'arte. Fiorilli's style differed from other commedia dell'arte players. He abandoned the mask and used the expressiveness of his face to enhance the comedy. His large eyes and eyebrows and long nose contributed to this effect, and he wore moustaches that framed the corners of his mouth and a thin, short goatee. These practices influenced Molière, and by most accounts the two actors developed a close relationship during the years they shared the same theaters. Some critics went so far as to accuse Molière of being Scaramouche's ape. One engraving shows Molière mimicking Scaramouche's grimaces with the help of a mirror. In the 1670s Fiorilli portrayed Scaramouche in London with great success. He was capable of deft physical farce, including dancing and acrobatics. It is reported that in his 80s he maintained the ability to simulate a kick to another actor's face. Fiorilli died in Paris and is buried in the Église Saint-Eustache.
In literature
, called Mezetin, wrote a "picaresque but untrustworthy biography": La Vie de Scaramouche, published in Paris in 1695 and Brussels in 1699.
A stage musical, Scaramouche - The Zany Commedia Musical, with book, lyrics and music by Stephen Lanigan-O'Keeffe, was premiered in Hoxton Hall, London, UK in 2007 and revised and restaged in 2011. The musical is a fusion of musical theatre and commedia dell'arte practices and treats Fiorillo's life and career as a parable on celebrity culture.