He was a son of regent Konoe Hisatsugu and a concubine. Motohiro was not considered a legitimate member at first, but his father Hisatsugu and his wife, Princess Shoshi, a daughter of Emperor Go-Mizunoo, had no child and Hisatsu died in Motohiro's childhood. Thus by an imperial order from Go-Mizunoo, Motohiro was installed in the Konoe lineage, and grew up under imperial protection. In 1654 he performed his genpuku ceremony and entered adulthood and therefore courtier life. In 1664 he married Princess Joshi, another daughter of Emperor Go-Mizunoo and his consort. With her he had a son, Iehiro, and a daughter, Teruko, who was a consort of Tokugawa Ienobu, the 6thshōgun of the Tokugawa shogunate.
After his entrance to the court, he served three emperors: Emperor Go-Mizunoo, Emperor Reigen and Emperor Higashiyama. Go-Mizunoo was his protector since his childhood, so his early career was prospective along with his noble lineage. But Emperor Reigen did not get along with the Tokugawa shogunate, and considered Motohiro sympathetic to the Shogunate, hence his career in Reigen's court was not as splendid. Tokugawa Tsunayoshi, the shogun at that time, was not warm to Ienobu at all, one of the candidates for his successor, hence also Motohiro, as the father-in-law of Ienobu. In Higashiyama's court, Motohiro however gained power again. He served as kampaku, the most powerful courtier from 1690 to 1703. After he quit, he had his supporters, including his own son, succeed the kampaku position respectively, and kept his influence. In 1704 the Tokugawa shogunate designated Ienobu, Motohiro's son-in-law, the successor of Tsunayoshi, hence the future shogun. His relation to the shogunate was therefore strengthened. Motohiro visited Edo twice, and was even welcomed to give political opinions, however it made the terms between ex-Emperor Reigen and him worse. Emperor Reigen even cursed him at Shimogamo Shrine, and accused him of being a "bad subject who privatizes and bends laws and justice" in his cursing prayer. However Motohiro was not always a supporter of Shogunate politics, and publicly objected to the Shogunate over some of their pressure on the imperial court. In 1722 he died and was buried at Daitoku-ji. He wrote a diary from 1655 until his death, later titled Diary of Lord Motohiro.
